Special thanks to Paul Köninger & Florian Kant

Homepage: http://2013.igem.org/Team:Freiburg/Team2

Lyrics:„Permanently Singing our CRISPR Song »

This morning I wake up in our lab – a pipet in my hands,
Hoping only that my little HEK cells haven’t died.
The incubator was not nice to me,
Temperature’s down to 25 degrees –
It seems that today I can hardly do transfections.

Let’s have a look at sequencing results – they must be great, yes.
My motivation’s far from the end and the data got sent.
But when I opened up the file, my friend –
Mr. Frameshift laughed at me and told me then:
„Baby, you should better stop performing Gibson Cloning! »

Why don’t you take our CRISPR Kit ?
Activation and Repression with Cas9 on stimulus!
Your genes need a refreshment now,
Get ready for Team Freiburg’s great fantastic show (WOW).
Our cloning methods failed so long,
But we’ll stay optimistic and know that it won’t go wrong.
This is how we struggle on,
Permanently singing our CRISPR song (3x)

Yesterday I worked till past 3 ` in the morning,
Picking clones from plate to plate and striking them out on more plates
I don’t wanna live that way,
DNA extractions make me faint.
Listening to my advisors curse,
and suddenly we see that someone else has
f****ing published first!

Why don’t you take our CRISPR Kit?
Activation and Repression with Cas9 on stimulus!
Your genes need a refreshment now,
Get ready for Team Freiburg’s great fantastic show (WOW).
Our cloning methods failed so long,
But we’ll stay optimistic and know that it won’t go wrong.
This is how we struggle on,
Permanently singing our CRISPR song (2x)

Damnit: My extraction failed
Damnit: Got no money left
Damnit: Contamination here
Damnit: Mutations everywhere

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